Cholinesterase variants: rapid characterisation by PCR/SSCP and evidence for molecular homogeneity.
نویسندگان
چکیده
منابع مشابه
Cholinesterase variants: rapid characterisation by PCR/SSCP and evidence for molecular homogeneity.
We have applied the technique of PCR-SSCP (polymerase chain reaction-single stranded conformation polymorphism) to characterise the molecular basis of cholinesterase deficiency and variants in a Jordanian family. PCR-SSCP proved to be a quick and sensitive method of screening cholinesterase variants in a clinical setting. An AG insertion at position 351 was found to cause a silent allele, for w...
متن کاملButyrylthiocholine Assay for Identification of Cholinesterase Variants
Two unusual variants of cholinesterase (acylcholineacylhydrolase, 3.1.1.8) have been recognized by the use of differential inhibitors (Kalow and Genest, 1957; Harris and Whittaker, 1961). A third variant, the silent gene, determines an absent or greatly reduced serum cholinesterase level in the affected homozygote (Liddell, Lehmann, and Silk, 1962). These variants are alleles, and the affected ...
متن کاملInhibition of the plasma cholinesterase variants by propranolol.
The inhibitory effect of (+/-) propranolol 1.69 x 10(-4)-1.69 x 10(-7) mol litre-1 on normal and atypical plasma cholinesterase variants was investigated. The atypical enzyme is less sensitive to inhibition by (+/-) propranolol or either of its enantiomorphs than the usual enzyme. Propranolol 8.45 x 10(-6) mol litre-1 was used as differential inhibitor of 643 plasma samples from individuals of ...
متن کاملPhantom Tracking Method, Homogeneity and Rapid Stabilization
In this paper we explain on various examples the “phantom tracking” method, a method which can be used to stabilize nonlinear control systems modeled by ordinary differential equations or partial differential equations. We show how it can handle global controllability, homogeneity issues or fast stabilization.
متن کاملRapid molecular techniques for detection of foodborne Bacillus cereus pathogen
Background and Objective: Bacillus cereus is responsible for several outbreaks of foodborne diseases. Therefore, the purpose of the study was to replace routine culture testing, by employing PCR directly from food to detect the bacteria and its enterotoxins. Methods: In the present study, a total of 75 Kibda sandwiches, Sausage sandwiches, Luncheon chicken, Luncheon meat and shawarma sandwiche...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Medical Genetics
سال: 1995
ISSN: 1468-6244
DOI: 10.1136/jmg.32.2.109